NF-κB activation is decreased after combinatorial stimulation with TNFα + FasL, but persists after IL-1β + FasL stimulation.

<p>Nuclear extracts of primary hepatocytes were prepared after treatment with IL-1β (20 ng/ml), TNFα (25 ng/ml), and FasL (50 ng/ml) for the indicated times. Additionally, cells were pre-treated for 30 min with the pan-caspase inhibitor Q-VD-OPh (20 µM) followed by IL-1β or IL-1β + FasL stimulation. Control cells remained untreated (lane 1). Equal nuclear protein amounts were analyzed for NF-κB DNA binding activity in an EMSA.•○ NF-κB-DNA complexes, ▪ non-specific binding to the probe, ◊ unbound oligonucleotide. The result of the EMSA was reproduced and one representative EMSA is shown.