Effect of human or mouse TNF-α against the pre-erythrocytic stage of PbA and Pyy265BY grown in Hepa 1–6 hepatoma cells or primary hepatocytes cultures.

<p>(A) Hepa 1–6 cells and (B) primary hepatocyte cultures were treated with 100 ng/ml of recombinant human or mouse TNF-α, 48 h prior to, at the time of, and then 3 h and 24 h after sporozoite inoculation. Cultures were stopped 48 h later. Data are presented as the mean (± SD) reduction in PbA liver schizont numbers at 48 h in triplicate experimental wells as compared to those enumerated in 6 control wells. In the control wells, there were 110.3±13.1 liver schizonts in Hepa1-6 and 602±83.4 in the primary mouse hepatocytes infected with PbA, and 25.5±3.6 liver schizonts in Hepa1-6 and 443.5±133.5 in primary hepatocytes infected with Pyy265 BY. * <i>p</i><0.05 versus control non-treated cultures (Kruskal-Wallis test, followed by Dunn test). The rate of infection for Pyy265 sporozoites was 0.02-.004% for Hepa1.6 cells and 0.1–2% for primary mouse hepatocytes. For PbA sporozoites, it was of 0.1–0.5% for Hepa1.6 cells and 0.5–1% for primary mouse hepatocytes. Data are representative of two independent experiments with similar results. ND, not done.