Heatmap representation of relative abundance of HCMV gene expression in infected glioma cells.

<p>All cells were infected with a strain of HCMV (MOI = 2). RNA was extracted at 72 hours p.i., 3 weeks p.i., and 5 weeks p.i. for gene expression analysis via SYBR green RT-PCR. Note that not all genes could be tested on each sample. The length of the heatmap represents the total number of genes tested for that particular sample. Ct values were normalized with housekeeping gene RPL13A to obtain ΔCt values, which were inputted into the Matrix2png program available at <a href="http://chibi.ubc.ca/matrix2png/index.html" target="_blank">http://chibi.ubc.ca/matrix2png/index.html</a> to generate the heatmap representation of relative transcript abundance. Green shows lower viral gene expression and red depicts higher expression. Black indicates that either the gene is not expressed or is below the limit of detection. A. U87 cells infected with TR virus. B. T98G cells infected with TR virus. C. 387 cells infected with TR virus. D. U87 cells infected with AD169 virus. E. T98G cells infected with AD169 virus.