Changes in cellular gene expression during HCMV infection.

<p><b>A.</b> Ribosome footprints and mRNA read densities (reads per kilobase million, RPKM) of well-expressed human transcripts after treatment with interferon (IFN), infection with inactivated virus (5hr UV) and across four time points during HCMV infection were calculated relative to expression in uninfected cells (mock). Shown is heat map of log₂ expression ratios after partitioning clustering. The ten main clusters are marked and for each of these clusters the pathway enrichment (Benjamini < 1E-5) is labeled on the left. <b>B.</b> Cells transfected with a control or siRNAs targeting different host genes were infected with the Merlin strain (MOI = 3). After 5 days supernatants were collected and viral titers were calculated by TCID50. Each experiment was performed in triplicates and results shown are representative. <b>C.</b> Samples from experiments detailed in (B) were analyzed by western blot for viral proteins expression from immediate early, early and late stages of infection (IE1/IE2, UL44 and pp28, respectively).