Hepatitis C virus infection induces up-regulation of potent oncogenes and angiogenic factors in Huh7.5 cells.

<p>Panel A. Huh7.5 cells were mock-infected or infected with HCV. Total RNA was isolated and the abundance of the indicated mRNAs was determined by qRT-PCR using <i>GAPDH</i> mRNA levels for normalization. The average +/− standard deviation of each transcript level from three infections is reported. Panel B. Total RNA from mock- or HCV-infected cells was fractionated using an antibody to the methyl guanosine cap structure and the relative abundance of each mRNA in the capped mRNA fraction was determined by RT-qPCR using oligo dT as a primer for the RT reaction to selectively amplify polyadenylated mRNAs. <i>GAPDH</i> mRNA was used as a reference for normalization and the average relative transcript abundance +/− standard deviation from three infections is shown. Panel C. Representative mRNA decay curves for the indicated transcripts as determined by qRT-PCR analysis of total RNA samples obtained at the indicated times post actinomycin D shut off of transcription. The average half-life +/− standard deviation from three infections is reported in the graph insets. Significance in each panel was determined by t-test with. * p ≤ 0.05, **p≤0.01, ***p≤0.005.