HCMV gene expression is detectable at 15 weeks p.i only in 387 GSC cells.

<p>A. 387 GSC cells infected with AD169 virus (MOI = 2) were analyzed for viral gene expression with SYBR Green RT-PCR at 72 hours, 3 weeks and every 2 weeks thereafter up to 15 weeks. B. Taqman validation of a subset of viral genes of AD169-infected 387 cells at 11 weeks p.i. Relative expression levels were obtained by normalizing viral gene expression with expression of cellular gene Rab14. Each sample was run in triplicate, bars represent S.D. C. Comparison of viral gene expression of AD169-infected glioma stem-like cells and cell lines at 72 hours and 5 weeks p.i. 3832 GSC cells were infected with AD169 virus (MOI = 2) and analyzed for viral gene expression with SYBR Green RT-PCR at 72 hours and 5 weeks p.i. These results are displayed alongside 72 hr and 5 week p.i. data for AD169-infected 387, U87, and T98G from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116178#pone-0116178-g001" target="_blank">Figures 1D–E</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116178#pone-0116178-g002" target="_blank">Figure 2A</a>.