Establishment of transgenic mice.

<p>(A) Genotyping using PCR method of transgenic mice. M, marker; +, positive control;-, blank; N, negative control; 1 and 2, positive transgenic mice; 3 and 4, negative mice. (B) Western blotting of calpastatin (CAST) in myocardial tissue from transgenic (TG) mice and wild-type (WT) littermates. Data are expressed as means ± SE. n = 6. **P<0.01 vs. WT group.