Generation of transgenic (TG) mice and their expression analyses.

<p>(A) Schematic map of the microinjected transgene consisting of the α-smooth muscle actin promoter and the mouse p122RhoGAP/DLC-1 cDNA. (B) Representative bands (131 bp) after genomic PCR and representative amplification curves for p122RhoGAP/DLC-1 and GAPDH in real-time PCR (40 cycles). (C) The gene expression of mouse p122RhoGAP/DLC-1 in various tissues of wild type (WT) (n = 3) and homozygous TG mice (n = 3). The ratio of p122RhoGAP/DLC-1 to GAPDH expression in the liver of WT mice was used as a reference (= 1), since it showed the lowest value among the tissues of WT mice. (D) Representative bands for mouse p122RhoGAP/DLC-1 protein in the pooled aortas (left panel) and densitometric analysis between WT and homozygous TG mice (right panel). The aortas from 4 mice in WT and TG.