Generation of CTRP2 transgenic mice.

<p><i>A</i>, Schematic of <i>Ctrp</i>2 transgene construct. HA epitope-tagged <i>Ctrp2</i> transgene is driven by the ubiquitous CAG promoter. <i>B</i>, RT-PCR analysis demonstrating the presence of <i>Ctrp2</i> transgene in Tg mice. <i>C</i>, Western blot analysis of CTRP2 in sera from WT and Tg mice. <i>D</i>, Quantification of relative band intensity (arbitrary unit) as shown in C. <i>E</i>, Western blot analysis of CTRP2-HA protein in mouse tissues.