Diet and metabolic state modulate the expression of CTRP2.

<p><i>A</i>, Quantitative real-time PCR analyses of <i>Ctrp2</i> expression in brown adipose tissue (BAT), visceral (epididymal fat pad), and subcutaneous (SubQ; inguinal fat pad) white adipose tissue from <i>ad libitum</i> fed (n = 10) or overnight (16 h) fasted male mice (n = 10). <i>B</i>, Quantitative Western blot analysis of CTRP2 serums levels in <i>ad libitum</i> fed or overnight (16 h) fasted male mice. Each lane represents data from an independent mouse. <i>C-D</i>, Real-time PCR (C) and Western blot (D) analysis of CTRP2 mRNA and serum levels in male C57BL/6 mice fed a high-fat diet (HFD) or a low-fat diet (LFD) for 12 weeks. Each lane on the immunoblot represents data from an independent mouse. Values shown are mean ± SEM. (n = 8–10 mice per group) *<i>p</i><0.05.