Genetic screen of transgenic mice derived from cytoplasmically microinjected eggs.

<p>A: Screen of transgenic founder mice by PCR. M: DL2000 DNA marker; 1–10: the founder mice derived from cytoplasmic microinjection with circular p2IS-UBC-eGFP plasmids (30 ng/µL) included into the native I-SceI nuclease digestive reaction system; 11–23: The founder mice derived from cytoplasmic microinjection with circular p2IS-UBC-eGFP plasmids plus NLS-I-SceI mRNA (30 ng/µL each). B: Screen of transgenic individuals of F1 offspring derived from transgenic founder mice by PCR. M: DNA marker; 1–8: Genomic DNA samples of F1 individuals. C: Genetic screen of transgenic founder mice by Southern blot assay. M: DNA molecular weight marker II; 1: plasmids; 2–7: genomic DNA samples of founder mice; 8: negative control (wild-type mouse genomic DNA). D: The transgenic mice exhibiting <i>in vivo</i> fluorescence derived from breeding between transgenic individuals over three consecutive generations. The arrow indicates the founder mouse detected to be transgenic by both Southern blot and PCR screen.