Delayed healing in NLRP-3 KO and caspase-1 KO mice.

<p>Images of day 5 wound cryosections stained with H&E for (A) wild-type, (B) NLRP-3 knockout and (C) caspase-1 knockout mice. Approximately one-half of the wound is shown. Note the reduced re-epithelialization and granulation tissue in the NLRP-3 knockout and caspase-1 knockout mice. Scale bar = 0.5 mm. Arrows indicate ends of migrating epithelial tongues. gt, granulation tissue; s, scab; ml, deep muscle. (D,G) Re-epithelialization was measured as the distance traversed by the epithelium divided by the distance between wound edges multiplied by 100 in H&E stained sections of the wound center. (E,H) Granulation tissue thickness was measured as the area of granulation tissue divided by the distance between wound edges in H&E stained sections of the wound center. (F,I) Trichrome staining was measured as percent area stained blue for collagen. Data presented as mean ± SE, <i>n</i> = 5–8 wounds per group. *<i>P≤</i> 0.05.