Reduced inflammatory cell accumulation in wounds from NLRP-3 KO and caspase-1 KO mice.

<p>Wounds were harvested on day 5 post-injury and cryosections from the center of each wound were stained with antibodies against Ly6G (neutrophils) and F4/80 (macrophages). (A-C) Representative images of Ly6G and (D-F) F4/80 stained sections, scale bar = 0.5 mm. (G-H) Ly6G and (I-J) F4/80 staining was quantified using the percentage of wound bed area stained. For each assay, digital images covering the majority of the wound bed (usually three images at ×20 magnification) were first obtained. The percent area stained in each image was then quantified by counting the number of pixels staining above a threshold intensity and normalizing to the total number of pixels. Data presented as mean ± SE, <i>n</i> = 7–8 wounds per group. *<i>P≤</i> 0.05. ^<b>†</b><i>P</i> = 0.057 for Ly6G staining (H) and <i>P</i> = 0.194 for F4/80 staining (J).