Injury boosts inflammatory cytokine expression in wounds of IL-4 Tg mice.

<p>Three mm full thickness excisional wounds were harvested at various time points and total RNA was extracted. After reverse transcription, expression of cytokine mRNAs was semi-quantified using real time PCR: a) IL-1β, b) IL-6, c) TNF-α, d) IL-4, e) IL-10, f) IL-13, g) IFN-γ, h) IL-12, i) TGF-β1, j) CXCL-2, k) CCL-2, l) NLRP3, * p<0.05, ^# p<0.01, and @ p<0.001 compared to the other group at the same time point, respectively. NS: normal or unwounded skin. The number of mice used at each time point was 5.