Transient hepatitis in LCMV-WE- infected C57BL/6J mice.

<p>Female C57BL/6J mice were divided into two groups and intravenously infected with either LCMV-WE or with LCMV-ARM, 1×10⁶ PFU in 0.3 ml of PBS. <u>Panel A.</u> IFA, staining for LCMV antigen (400x magnification); H&E, staining for hematoxylin and eosin (200x); 4HNE, IHC with a monoclonal anti-4HNE IgG antibody as a marker of oxidative damage and lipid peroxidation (200x). Arrows point to positive staining (IFA and 4HNE) or inflammatory foci (H&E). Insets (1000x magnification) depict inflammatory cell recruitment in LCMV-infected livers. *, numbers above IFA staining panels are viral titer values for LCMV-WE infected liver extracts in PFU/g; ND, not determined, lower than detection limit (<1.2 log₁₀ PFU/g, see details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0122839#sec009" target="_blank">Results</a>). <u>Panel B.</u> Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined as described in Materials and Methods. Data are means ± SEM (n = 4–7).^a, <i>P</i><0.05 compared with sham infection;^b, <i>P</i><0.05 compared with LCMV-ARM infection.