Evident LCMV-induced hepatitis with overt clinical symptoms, rise in serum cytokines and intensified F4/80+ cell staining.

<p>During the course of LCMV Cl13 infection mice were weighed (A) and clinically scored using predefined criteria (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0166094#pone.0166094.t001" target="_blank">Table 1</a>) (B). At regular intervals mice were sacrificed to determine the intrahepatic LCMV viral load (C). Serum was collected at different time points to measure ALT (D) and cytokines TNF, IFNγ, CXCL1, and IL10 (D) using a multiplex assay. X-axis shows days post infection (A-D). Error bars indicate mean ± SEM (A-D). ND, not determined (C). F4/80 IHC staining was performed at indicated time points to characterize the presence, morphology and localization of F4/80+ cells within the liver (E). Insert at day 0 shows non-primary control staining of mouse spleen (E). Scale bar indicates 100 μm (E).