Immunolocalization of Kif5b in mouse kidney PT (A-C), TAL (D-F), CD (G-I) and DCT (J-K), using antibodies against specific markers for each segment.

<p>Kif5b was expressed in TAL (THP-positive tubules) and DCT (NCC-positive tubules), but not in PT (LTL-positive tubules) or CD (AQP2-positive tubules). (L) The diagram shows the selective and asymmetric expression pattern of Kif5b in renal tubules of adult mice. Red, Kif5b; orange, LTL; yellow, THP; green, NCC; light blue, AQP2; blue, nucleus. Only cells from one side of the tubules are presented and the other side is represented by a dotted line. Arrows represent the direction of urinary flow. Cortex and medulla are separated by a dashed line. G, glomerulus; PT, proximal tubule; TDL, thick descending limbs of Henle; TS, thin segment; TAL, thick ascending limbs of Henle; DCT: distal convoluted tubule; CD, collecting duct. Images are representative of kidney tissue sections from three mice. Scale bar = 50 μm.