Nephron number is decreased in both <i>Kif3a^−/−UB</i> and <i>Kif3a^−/−MM</i> kidneys.

<p>(A–C) Nephrogenic precursor structures, marked by expression of NCAM (red color, marked by arrows), in <i>Kif3a^−/−UB</i> mice (B) and <i>Kif3a^−/−MM</i> mice (C) versus control mice (A). (D–F) Identification of glomeruli via expression of WT1 (green color, marked by arrow). Collecting ducts are identified by expression of cytokeratin (red color). (G–I) Identification of WTI-positive structures (red color, marked by arrow) at P0. Proximal tubules are identified by expression of LTL (green color, marked by arrowhead). (J, K) Quantification of the number of NCAM–positive and WT1-positive structures demonstrates a decreased number in <i>Kif3a^−/−UB</i> and <i>Kif3a^−/−MM</i> mice. (***, P<0.001; **, P<0.01; *, P<0.05). Scale bars: 25 micrometer.