Spatiotemporal expression pattern of Kif5b in mouse kidney.

<p>(A, B) Kif5b was widely distributed inside renal tubular epithelial cells of newborn mice. (C-H) Kif5b was gradually restricted to epithelial cells in DCT and TAL during postnatal kidney development. (I) Quantitative analysis of the relative optical density values for cells in renal tubules. Kidney sections were analyzed using ImageJ software to determine the mean optical density values per cell of positive-labeled tubules in the kidney. The relative mean optical densities of cells in the tubular structures compared with that in the DCT were calculated. Bars represent the means ± S.D. (n = 10 for each tubular structure from three mice were measured and analyzed). One-way ANOVA was performed to compare relative intensity levels of different tubular structures at indicated stages. * <i>P</i><0.05 versus DCT. (J) Kif5b was evenly distributed in the cytoplasm of renal epithelial cells in newborn mice. (K) Kif5b was asymmetrically distributed in the basolateral domain in renal tubular cells of adult mice. Lu, lumen; PCT, proximal convoluted tubules; TAL, thick ascending limbs of loops of Henle; DCT, distal convoluted tubule; CD, collecting duct. Images are representative of kidney tissue sections from three mice. Scale bar: (A-H) 50 μm, (I, J) 10 μm.