AAV-HBV-mediated efficient gene transduction <i>in vivo</i>.

<p>(a) Serum HBsAg was measured in a quantitative ELISA and the average ng/ml serum was plotted at the indicated times p.i.. Data represent the mean ±SEM. (b) Serum HBeAg was measured in a quantitative ELISA and the average NCU/ml serum was plotted at the indicated times p.i.. Data represent the mean ±SEM. (c) Immunohistochemical staining of HBsAg and HBcAg in liver sections. Staining was performed in tissue sections from four animals per group. Representative sections are shown. (d) Quantification of the immunohistochemical data shown in (c).Ten random fields were selected per slide and the percentages of HBsAg- and HBcAg-positive hepatocytes were quantified using Image-Pro Plus (Media Cybernetics, Rockville, MD). Statistical analyses were performed using two-way analysis of variance. HBV(-) mice, PBS injected mice.