Serum viral DNA and the expression of viral proteins in mice after HI with pWHV-HBV-Sa.

<p>(A) Six mice (Sa1-Sa6) were hydrodynamically injected with pWHV-HBV-Sa, while four mice (H1.3–1 to H1.3–4) were hydrodynamically injected with pBS-HBV1.3. Mouse sera were collected at the indicated time points after HI and subjected to HBsAg ELISA. The results were read at OD 450 nm. The cut off value was set as 0.1 and indicated by the dotted line. (B) Detection of serum encapsidated viral DNA by PCR. PCR products were visualized by agarose gel electrophoresis. Neg.: Sera from two mice (N1, 2) challenged by saline. (C) Detection of hepatic WHcAg expression by ICS at days 0, 10, 42 and 98 after HI with pWHV-HBV-Sa. A set of representative samples was shown. Magnification: 200×.