HBV expression in NOG mice transfected with pHBA1.2 or pHBC1.2.

<p><b>(A-C)</b> NOG mice were transfected with pHBA1.2 or pHBC1.2 by hydrodynamic injection and analyzed 1 and 3 days after transfection. <b>(A)</b> Representative images of immunohistochemical staining for HBcAg in liver sections 1 day after hydrodynamic injection. The number of HBcAg-positive cells in liver sections and the plasmid levels in the livers (N = 9–10). <b>(B)</b> Serum HBV DNA levels, <b>(C)</b> HBsAg levels and HBeAg levels 1 day after injection (N = 9–10) and 3 days after injection (N = 4). <b>(D-E)</b> NOG mice were transfected with pHBA1.2 or pHBC1.2, and blood was collected at the indicated time points. Open squares stands for mice with pHBA1.2, while closed triangles stands for mice with pHBC1.2. <b>(D)</b> The serum levels of HBV DNA (N = 7–10). <b>(E)</b> The serum levels of HBsAg and HBeAg (N = 7–10). <b>(F-G)</b> NOG mice were transfected with pHBA1.2 or pHBC1.2 and analyzed at the indicated time points post-transfection. Gray bars stands for mice with pHBA1.2, and black bars stands for mice with pHBC1.2. <b>(F)</b> The number of HBcAg-positive cells in the liver sections was determined by immunohistochemical staining. They were subjected to the Mann–Whitney U-test. (N = 5–7). <b>(G)</b> CccDNA and plasmid DNA levels in livers at each time point (N = 4–7). *, P < 0.05.