Western blot analysis of the recombinant PS proteins using the PS K196E mutation-specific monoclonal antibody.

<p>FL-PS-K and FL-PS-E were separated by SDS-PAGE under reducing conditions, transferred to a membrane, and incubated with the anti-His tag antibody (A) or the PS K196E mutation-specific monoclonal antibody 15C8 (B), and then with HRP-labeled anti-mouse IgG. The proteins were visualized using a chemiluminescent substrate. The molecular weights are shown on the left.