Detection of Mos (A), Emi2 (B), Cyclin E (C) and Cdk2 (D) by Western blotting in PS and RS.

<p>Fifty µg of PS and RS were resolved by SDS-PAGE (10% gel). For each protein are presented (i) the Western blot (WB) highlighting the specific bands: 39 and 43 kDa for Mos, 71 kDa for Emi2, 51 kDa for Cyclin E (40 and 33 kDa are low molecular weight derivatives) and 34 kDa for Cdk2, and (ii) the staining of the nitrocellulose membranes with Ponceau Red S (NM-PR) assessing the blotting of proteins. The molecular weights of each specific band are indicated on the left of the WB. Locations of molecular mass standards are shown on the left of NM-PR.