Recognition of hybrid proteins by antibodies against PspA and Pds.

<p>Recombinant proteins were separated by SDS-PAGE and transferred to PVDF membrane - A) rPspA2 and rPspA2-PdT; B) rPspA1 and rPspA1-PlD1; C) rPspA1 and rPspA1-PlD2; D) rPdT and rPspA2-PdT; E) rPlD1 and rPspA1-PlD1; and F) rPlD2 and rPspA1-PlD2. The membranes were incubated with anti-rPspA2 (A) anti-rPspA1 (B and C) or anti-rPly (D, E and F) followed by incubation with anti-mouse IgG conjugated with HRP. Detection was performed with an ECL kit (GE Healthcare). Molecular mass markers (kDa) are indicated on the left.