Production and reaction of recombinant proteins rPDHA-D and corresponding polyclonal guinea pig antisera.

<p>EK-LIC vector and <i>E</i>. <i>coli</i> BL21(DE3) were used to produce recombinant proteins. (A) Proteins were purified, concentrated, separated by SDS-PAGE and stained with Coomassie. (B) Western blot reaction of total proteins of <i>M</i>. <i>pneumoniae</i> M129 with antisera to rPDHA-D. (C) Results of ELISA experiments to analyze the reactivity of antisera to total proteins and to PDH subunits A-D with whole antigen of <i>M</i>. <i>pneumoniae</i> M129. Data represent means and standard deviations of eight parallels.