Dissemination of primed antigen-specific T cells after nasal immunization with OVA and CpG ODN 1826.

<p>CD4+ T cells, isolated from OT-II mice, were labeled with CFSE and adoptively transferred into recipient C57BL/6J mice. Twenty-four hours later, recipient mice were immunized with OVA (25 <i>μ</i>g/mouse) and CpG ODN (20 <i>μ</i>g/mouse) by the nasal route. CD4+ T-cell proliferation was assessed in cervical (CLN), iliac (ILN), mesenteric (MLN) lymph nodes and spleen (SPL) by CFSE dilution at different time points following immunization.(A) OVA-specific proliferation of OT-II CD4+ T cells was analysed in different lymphoid organs three days after immunization. Histograms are gated on CD4+CFSE+ population, with light scatter properties of lymphocytes. The number of generation is reported for each peak. (B) Graphs reporting the percentages of CD4+ CFSE+ T cells for every cell generation in CLN, ILN, MLN and SPL 0, 57, 72, 84 and 96 after immunization. Values are reported as mean ± standard error of the mean (SEM).