Intranasal administration of OVA with the adjuvant CT results in an increased proliferation of CFSE labeled OT-II cells <i>in vivo</i>.

<p>CFSE -labeled OT-II cells (5×10⁶) were adoptively transferred into C57BL/6 mice. One day after transfer, mice were i.n immunized with either PBS, CT, OVA or CT with OVA (n = 3 per group). Five days after immunization, MeLN (pooled), CLN and MLN were removed and analyzed using flow cytometry to determine the presence and number of divisions undergone by CFSE OT-II cells <i>in vivo</i>. Propidium iodide (PI) was used to exclude dead cells from the analysis. (A) Shown is a representative histogram from one individual animal of each group. The number of dividing CFSE-labeled, PI negative, Vα2⁺, CD4⁺ T cells in the MeLN (B) and CLN (C) was determined per 5×10e⁵ lymphocytes. Data are representative of 3 individual experiments. *denotes <i>P</i><0.05, Students’ t-test.