Oral antigens are presented in the draining lymph nodes of both healthy and DSS treated mice.

<p>A) OVA presentation in the gastrointestinal tract was visualized by the proliferation of adoptively transferred, CFSE labeled, OTII T cells. Representative FACS dot plots displaying OTII T cell proliferation within the mLNs of both healthy and DSS-treated mice after oral gavage of saline, “no antigen” or oral gavage with OVA, “ovalbumin”. The loss of CFSE intensity is an indication of dividing T cells. B) Percent proliferated OTII cells found within isolated mLNs. C) Percent proliferated OTII cells in the non-local, axillary lymph nodes. Results for (B) and (C) are expressed as mean + SEM, N = 4 mice per group, pooled from two independent experiments. * P < 0.05; ** P < 0.01.