Antigen-specific T-cell distribution in draining, distal lymph nodes and spleen following vaginal or nasal immunization.

<p>CFSE-labelled OT-II CD4⁺ T cells were transferred into recipient C57BL/6J mice. Twenty-four hours later, recipient mice were immunized with OVA (25 µg/mouse) and CpG ODN 1826 (20 µg/mouse) by the vaginal or nasal route. The average number of OVA-specific CD4⁺ T cells was calculated in draining, distal lymph nodes and spleen of mice 57 hours following vaginal or nasal immunization or in naïve mice. Here the ratio between the absolute number of OVA-specific CD4⁺ T cells detected in lymphoid organs of mice immunized by the vaginal or nasal route <i>versus</i> naïve mice is reported.