Antigen uptake by DCs following vaginal or nasal immunization.

<p>C57BL/6 mice were immunized by the vaginal or nasal route with OVA Alexa Fluor 647-conjugate (25 µg/mouse) and CpG ODN (20 µg/mouse). Groups of animals were sacrificed 0, 12, 24 and 72 hours after mucosal immunization and iliac lymph nodes (considered draining after vaginal and distal after nasal immunization respectively), cervical lymph nodes (distal after vaginal and draining after nasal immunization respectively) and spleen were collected. DCs positive for fluorescent OVA were analysed by flow cytometry. Shown is the percentage of OVA-Alexa Fluor 647 positive cells among total CD11c⁺ MHC class II⁺ cells.