Effects of AR antagonists are blunted in 22RV1 cells.

<p>(A) Luciferase activity measured in 22RV1 cells transfected with ARE-luc reporter and treated +/- DHT (1nM), +/- Flutamide (1μM), or +/- MJC13 (30μM) overnight, with quantities of plasmids as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0137103#pone.0137103.g001" target="_blank">Fig 1A</a>. All data are representative of at least three independent experiments with similar results. All values represent the mean ± SD of duplicate samples. (B-C) qPCR analysis of 22RV1 cells extracts treated +/- DHT (1nM), +/- Flutamide (1μM), or +/- MJC13 (30μM) for 24 hours. The data are representative of at least three independent experiments. All values represent the mean ± SD of triplicate samples. Genes are TMPRSS2 (B) and PSA (C).