Transactivation of AR promoter by FGF19 treatment depends on the integrity of TBE sites.

<p>Hep3B cells were transiently transfected with the empty luciferase reporter plasmid (pGL3), with the wild type AR promoter luciferase reporter construct (ARwt pGL3) and with the three AR promoter constructs in which the three TBE sites had been individually mutated (ARTBE1mut pGL3, ARTBE2mut pGL3 and ARTBE3mut pGL3). Cells were treated as indicated with FGF19 (50 ng/mL, 12 h) and luciferase activity was measured as described in Methods. *<i>P</i><0.05 <i>vs</i> untreated ARwt pGL3 transfected cells. §<i>P</i><0.05 <i>vs</i> ARwt pGL3 transfected cells treated with FGF19. AU: arbitrary units.