Stimulation of cell-based reporters by sulforaphane, oltipraz and stressors.

<p>S2 cells transiently transfected with TRE-fluc, ARE-fluc or mRE-fluc plasmids, as indicated, were exposed to sodium arsenite (NaAsO₂), Diethyl Maleate (DEM) or oltipraz. The firefly luciferase activity was measured 24 hrs after drug treatment and normalized to renilla luciferase activity expressed from a co-transfected Act5C renilla luciferase construct. Note that both TRE and ARE reporters respond to the stressors, Arsenite and DEM, but only ARE-fluc responds to Oltipraz. The mRE reporter responds neither to oltipraz nor to the stressors. The activity of mRE-fluc reporter under control conditions was set to 1. Error bars indicate standard deviation of triplicate measurements.