Luciferase reporter assay using the <i>BMP4</i> promoter region as a cis element.

<p>Activity of firefly luciferase expressed from the reporter vector was normalized with <i>Renilla</i> luciferase, and shown as arbitrary units (mean ± SEM). Experiments were repeated three times with independent transfections, and for each experiment luciferase activity was measured in triplicate. Protein samples were prepared from aliquots of transfected cells, and ectopic PAX9 expression was verified by immunoblotting. An alleviating effect of P20L on transactivation was supported statistically, while that of A240P was not, as shown above the graph.