Figure 2

<p>A. Comparison of firefly luciferase reporter plasmids. COS-7 cells were cotransfected with the indicated luciferase reporter plasmid and pSVARo expressing WT hAR. The cells were treated with DHT and firefly luciferase activity was determined. The results were calculated as the fold stimulation of a given reporter plasmid compared to untreated cells transfected with the same plasmid. Values are means of a minimum of three independent experiments performed in quadruplicate ± SEM. B. Western blotting analysis of hAR expressed by mutated pSVARo plasmids. COS-7 cells were transiently transfected with plasmid encoding the indicated mutation, and cell lysates were analyzed for hAR by western blotting. Lysate from untransfected cells (UT) was used as the control.