Endogenous AR transcriptional activity in LacZ and ARΔTR lentiviral vector transduced CWR-R1 cells.

<p>CWR-R1 cells were transiently transfected with the MMTV-luciferase reporter and assayed for luciferase activity in the presence and absence of DHT. In the presence of 0.1 nM DHT, CWR-R1 cells expressing the LacZ transgene demonstrated an increase in MMTV-luciferase reporter activity compared to control cells without DHT. Expression of ARΔTR reduced MMTV-luciferase reporter activity in the absence or presence of 0.1 nM DHT compared to LacZ-transduced control cells. Columns represent mean luciferase activity in relative light units from 3 independent experiments; bars ± standard deviation.