Additional file 9: Figure S8. of Tools and best practices for data processing in allelic expression analysis

QC measures reduce overdispersion in technical replicates when testing for allelic imbalance using a binomial test. Variance of allelic ratios as a function of total read counts, calculated as the mean at a given SNP from a Geuvadis individual with eight technical replicates (grey) with (b) or without (a) accounting for duplicate reads and overlapping read mates. The lines denote locally weighted smoothing of observed data (black) and theoretical variance for binomially distributed data (red). (TIFF 3209Â kb