<i>Ripk3</i>^-/- mice show impaired collagen deposition.

<p>Skin samples from the wound sites of WT and <i>Ripk3</i>^-/- mice were collected at the indicated days for evaluation of collagen deposition by Masson-Trichrome staining and qPCR (n = 5 mice per group). Representative images of Masson-Trichrome stained skin sections (40× magnification) from day 7 (A, B) and day 14 (C, D) wounds from WT (A, C) and <i>Ripk3</i>^-/- (B, D) mice are shown. Collagen is stained blue. Angiogenic sites are depicted by arrows; ep, new epithelium; wb, wound bed. Scale bar = 500 μm. (E) The expression analysis of collagen type 1 α 1 (<i>Col1a1</i>) mRNA by qPCR. Data expressed as mean ± SEM (n = 5 mice per group) and compared by one-way ANOVA using SNK test. *<i>P</i> < 0.05 versus WT at the indicated day.