<i>Ripk3</i>^-/- mice show altered early neutrophil infiltration and inflammation.

<p>(A-E) Skin samples from the wound sites of WT and <i>Ripk3</i>^-/- mice were collected at days 1 and 3 for immunohistochemical staining with Gr-1 antibody. Representative images of Gr-1 stained skin sections (200× magnification) from day 1 (A, B) and day 3 (C, D) wounds from WT (A, C) and <i>Ripk3</i>^-/- (B, D) mice and quantitative analysis of neutrophil infiltration expressed as number of neutrophils/field (E) are shown. Neutrophils are stained brown. Scale bar = 100 μm. (F-I) Skin samples from the wound sites of WT and <i>Ripk3</i>^-/- mice were collected at the indicated days. RNA was extracted and cDNA was prepared from these samples for gene expression analysis of IL-6 (F), KC (G), IL-1β (H) and TNF-α (I) by qPCR. Data expressed as mean ± SEM (n = 3 (E) and 5 (F-I) mice per group for each time point) and compared by one-way ANOVA using SNK test. *<i>P</i> < 0.05 versus WT at the indicated day.