Increased skin collagen accumulation in Nab2 ^−/− mouse.

<p>Skin from Nab2^−/− mice and wildtype mice was harvested. A. Tissues were stained with hematoxylin and eosin (a–d) or picrosirius red (e,f). Original magnification ×100 (a, b), ×400 (c–f). B. Immunofluorescence using antibodies to α-smooth muscle actin (green). Nuclei were identified by DAPI (blue). Original magnification ×100 (a, d), ×200 (b, c, e, f). C. Total RNA was harvested and was subjected to real-time qPCR analysis. Results, expressed relative to 18S, are the means±S.D. of triplicate determinations from a representative experiment.