Pulmonary metastasis of B16V cells and analysis of the primary tumors.

<p>(<i>A</i>) Dissected control and B16V^shUst(16) primary tumors (left panel) and their weight (right panel) measured after 15–21 days of inoculation (three independent experiments n = 13–15). (<i>B</i>) Macroscopic evaluation of lungs of mice after 6–7 weeks of primary tumor dissection. Three representative pictures of each control and B16V^shUst(16) inoculated mice. (<i>C</i>) Representative blots of control and B16V^shUst(16) primary tumor lysates for Ust, α5 integrin and β-actin as loading controls.