MS-1 mAb suppressed spontaneous pulmonary metastasis and tumor growth <i>in vivo</i>.

<p>A to D, CHO/Aggrus cells were xenografted in BALB/c-<i>nu/nu</i> mice. Antibodies (30 µg/mouse) were intravenously injected on the following day of tumor inoculation and repeated two more times every fourth day (arrow heads). Tumor volume was calculated as described in Materials and Methods. All data are shown as means ± SDs (n=5). **<i>P</i> < 0.01 by the Mann–Whitney <i>U</i> test (A). Representative pictures of CHO/Aggrus tumor-bearing mice on day 18 are shown (B). After 29 days of tumor inoculation, spontaneously pulmonary metastatic foci were counted. The numbers of metastatic foci are shown. Bars, mean (n = 5). **<i>P</i> < 0.01 by the Mann–Whitney <i>U</i> test (C). Representative pictures of the lungs and lung surface metastatic foci are shown (D). E, a lung squamous cell line PC-10 (left) and a lung adenocarcinoma cell line A549 (right) cells were treated with control mouse IgG (gray area) or MS-1 mAb (bold lines). Aggrus expression was detected by flow cytometry. F and G, PC-10 cells were xenografted into BALB/c-<i>nu/nu</i> mice. Antibodies were intravenously injected on 1, 4, 7, and 11 days after tumor inoculation (arrow heads). Tumor volume was calculated as described in Materials and Methods. All data are shown as means ± SDs (n=8). **<i>P</i> < 0.01 by the Mann–Whitney <i>U</i> test (F). Representative pictures of the PC-10 tumor-bearing mice on day 18 are shown (G).