P-selectin deficiency inhibited B16 lung metastasis in P-sel^−/− mice.

<p>(A) Gross anatomy of B16 lung metastases in C57 and P-sel^−/− mice. (B) The quantification of the metastatic foci indicated that P-sel^−/− mice developed fewer metastatic foci on the lung surface than C57 mice. (C) HE staining of the metastatic tumors confirmed their homology with B16 cells, and the metastatic foci quantification in the bulk tissue indicated that P-sel^−/− mice developed fewer metastatic and micrometastatic foci than C57 mice. Scale bars: 100 µm. (D) Fontana-Masson melanin staining of B16 cells and lung metastases. Scale bars: 20 µm. **P<0.01.