Sensitivity and specificity of the <i>BmHha</i> I colorimetric NINA-LAMP assay with neutral red dye.
- Catherine B. Poole (111223)
- Zhiru Li (295010)
- Andy Alhassan (225478)
- Dylan Guelig (666473)
- Steven Diesburg (802744)
- Nathan A. Tanner (111229)
- Yinhua Zhang (111234)
- Thomas C. Evans Jr. (111240)
- Paul LaBarre (221735)
- Samuel Wanji (216392)
- Robert A. Burton (3749677)
- Clotilde K. S. Carlow (111245)
Publication date
February 2017
DOIAbstract
<p>Comparison of sensitivity using <i>Bst</i> DNA polymerase, Large Fragment (wt <i>Bst</i> LF), <i>Bst</i> 2.0 DNA polymerase (<i>Bst</i> 2.0) or <i>Bst</i> 2.0 WarmStart DNA polymerase (<i>Bst</i> 2.0 WS) on a 10X serial dilution of genomic <i>B</i>. <i>malayi</i> DNA (A). Species-specificity using 100 pg of various genomic DNAs (B). Detection of <i>B</i>. <i>malayi</i> DNA in mosquitoes (C). Species names are abbreviated as follows: <i>B</i>. <i>malayi</i> (Bm), <i>W</i>. <i>bancrofti</i> (Wb), <i>O</i>. <i>volvulus</i> (Ov), <i>Homo sapiens</i> (Hs), <i>Simulium vittatum</i> (Sv) and <i>Aedes aegypti</i> (Aa). The non-template controls (NTCs) contain water.</p
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