Sensitivity of the LAMP assay in simulated human urine samples artificially contaminated with DNA from <i>S</i>. <i>haematobium</i>.

<p>(A) Sensitivity assessment of LAMP when performing the DNA extraction with the i-genomic Urine DNA Extraction Mini Kit (Intron Biotechnology, UK) from serial dilutions of <i>S</i>. <i>haematobium</i> genomic DNA. (B) Sensitivity assessment of LAMP when performing the DNA extraction with a simple heating method from serial dilutions of <i>S</i>. <i>haematobium</i> genomic DNA. Lanes M: 50 bp DNA ladder (Molecular weight marker XIII, Roche); lanes Sh: genomic DNA from <i>S</i>. <i>haematobium</i> (1 ng); lanes 10⁻¹–10⁻¹¹: 10-fold serially dilutions; lanes N: negative controls (no DNA template).