Sensitivity and specificity of the <i>WbLDR</i> colorimetric NINA-LAMP assay using <i>Bst</i> 2.0 WS.

<p>(A) LAMP reactions incubated in the NINA heater exhibit the same sensitivity as those incubated in a qPCR machine with DNA amplification monitored in real time using a dsDNA binding dye. Genomic <i>W</i>. <i>bancrofti</i> DNA dilutions equivalent to 1/5 mf (1), 1/50 mf (2), 1/500 mf (3), 1/5000 mf (4), 1/50,000 mf (5), 1/500,000 mf (6), 1/5,000,000 mf (7) and H₂O (8) were used as template. The end point color change of the LAMP reactions incubated in the NINA heater are shown in the upper panel. Real time amplification curves and end point color change of LAMP reactions are shown in the lower panel. (B) Species-specificity using 100 pg of various genomic DNAs as template. (C) Detection of <i>W</i>. <i>bancrofti</i> DNA in blood samples from 8 different LF positive patients (S1-S8). Species names are abbreviated as follows: <i>W</i>. <i>bancrofti</i> (<i>Wb</i>), <i>B</i>. <i>malayi</i> (<i>Bm</i>), <i>O</i>. <i>volvulus</i> (<i>Ov</i>), <i>Aedes aegypti</i> (<i>Aa</i>) and <i>Homo sapiens</i> (<i>Hs</i>). All experiments were performed in triplicate. One representative of each is shown.