Sensitivity and specificity of the <i>OvGST</i>1a colorimetric NINA-LAMP assay using <i>Bst</i> 2.0 WS.

<p>Sensitivity comparison of neutral red and phenol red dyes on a 10X dilution series of genomic <i>O</i>. <i>volvulus</i> DNA (A). Species-specificity using 1 ng of various genomic DNAs (B). Detection of <i>O</i>. <i>volvulus</i> in black flies (C). Species names are abbreviated as follows: <i>O</i>. <i>volvulus</i> (Ov); <i>W</i>. <i>bancrofti</i> (Wb); <i>Loa loa</i> (Ll); <i>B</i>. <i>malayi</i> (Bm); <i>Aedes aegypti</i> (Aa); <i>Simulium vittatum</i> (Sv); <i>Simulium squamosum</i> (Sa) and <i>Homo sapiens</i> (Hs). NTC = Non-template control.