Species-specificity of <i>Hha</i> I LAMP assay.

<p>(A) Each curve represents the calculated average of triplicate turbidity curves generated with various genomic DNAs (0. 1 ng) using <i>Bst</i> 2.0 DNA polymerase without loop primers. Turbidity was observed using <i>B. malayi</i> or <i>B. timori</i> DNA. (B) As a positive control, an actin gene fragment was PCR amplified from <i>B. malayi</i> (Bma), <i>D. immitis</i> (Dim), <i>O. volvulus</i> (Ovo), the mosquito <i>Aedes albopictus</i> (Aal), <i>W. bancrofti</i> (Wba), human (Hsa) and <i>B. timori</i> (Bti) DNAs using degenerate primers. Agarose gel showing amplification of a 244 bp fragment of the actin gene. The 100 bp DNA Ladder (New England Biolabs) was used as the molecular weight marker (MWM). Water was used in the non-template controls (NTC) in (A) and (B).