Additional file 1 of Multifunctional Fe-based coordination polymer nano-bomb modified with β-lapachone and CaO2 for targeted tumor dual chemodynamic therapy with enhanced ferroptosis and H2O2 self-supply

Additional file 1: Figure S1. XRD patterns the HCF upon treatment in different solvents for 7 days. Figure S2. Particle size changes of FCP, CF and HCF nanosystem. Figure S3. The amount of GSH in the supernatant after 24 hours of reaction between HCF@β-lap at different concentrations and 10 mM GSH in mixed solution. Figure S4. Intracellular ROS levels and cell membrane staining. Figure S5. O2 concentration measurement in the presence of the weak acidic microenvironment. Figure S6. CLSM images of hypoxia level in 4T1 cells after PBS and HCF@β nanosystem treatment for 4 h. Figure S7. Relaxation rates r1 and r2 of solutions of the HCF@β-lap. Figure S8. 4T1 cell live/dead staining after various treatments. The red signal denoted dead cells, while the green signal denoted live cells. Figure S9. Quantitative apoptosis statistics of 4T1 cells induced by difference treatments after 24 h of incubation. Figure S10. The corresponding quantitative analysis of intracellular Ca2+ concentration via Fluo-4, AM staining. Figure S11. The relative fluorescence density analysis of 4T1 cells on mitochondrial damage after administration for 12 h using JC-1 probe. Figure S12. Changes in mice body weight during administration. Figure S13. Tumor-related quantitative analysis of TUNEL and Ki67 immunofluorescence images. Figure S14. The blood biochemical levels and hematological indices of mice after 7 day of administration. Figure S15. The pharmacokinetics of HCF@β-lap and CF@β-lap